SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Using the. edu, 814-863-3503. Five preferred detection modes. 1. We therefore gathered salient. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Compact and tunable absorbance microplate readers DATA SHEET. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. Update Driver. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and wester. The SpectraMax M5 Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Microplate Reader Shelf. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. com 10. Gemini EM. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. View the Molecular Devices website. Description 1. Shake. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. com 4. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. Category 2 and 3 microplate readers use gradient heating as well. M5 and M5e Multi-Mode Microplate Readers User Guide SpectraMax M3, M4, M5. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. SpectraMax® microplate readers: reader check and error codes Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader,. Choose from a three- (M3), four- (M4), or Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. #3635 or Greiner cat. View online or download Molecular devices SpectraMax M5e User Manual. High-performance. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. You can also interpret your results using integrated data analysis software. It does not work as expected. Prepare your iD series instrument for shipment. The optical systems . As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Features. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . After the plate is read, data will appear in the plate section: 11. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. free tracer decreases, resulting in an. 1. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. 315 can't find zero order. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. 3: The cuvette chamber. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. This five-mode microplate reader with automatic filter identification. SpectraMax Paradigm Multi-Mode Safety Guide SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. The chamber. Manufacturer: Molecular Devices Model: SpectraMax Plus 384 The SpectraMax ® Plus 384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. To assess the sensitivity of each microplate reader, a plate was set up with 32 wellsThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader,. Maximum signal with lowest background. Choose from a three- (M3), four- (M4), orThey include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate. Read More. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Read More. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Description. Following treatment the samples were assayed for protease activity in triplicate using the Pierce Fluorescent Protease Assay Kit (Thermo Scientific, Waltham USA),. Please refer to the Obsolete Microplate Reader Models for more information. Accordingly there are different configurations possible. Wide range of applications. Plate Shaking Linear, Orbital Slow, and Orbital Fast. easy to use and read multiple plates very quickly. For SpectraMax L or LMax II, LMax II. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Temperature Regulation. 8. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. 12. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated. Cells in each. Microplate Readers. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. Published Dec 16, 2020 | Updated Dec 16, 2020. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. N. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Fluorescein 0. SpectraMax i3 reader settings for IMAP PDE assays. 2. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate. The Spectr. Choose from a three- (M3), four-. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. SpectraMax M5 Multi-Mode Microplate Reader Parts Repair LA Pickup. Page 28 Chapter 5: Read Modes and Read Types 1. Delivers single mode reader performance in one multimode reader package. Choose from a three- (M3), four- (M4), orThe SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Figure 1. The plate reader can measure multiple readouts, including UV. techsupport. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. Performing an enzyme dilution series verifies the enzyme and assay performance and allows calculation of the EC 80 concentration of enzyme that will be used for inhibitor screening assays. 1. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. flexibility of monochromator-based systems. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. By offering two new configurations of multi-mode readers that deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an even greater combination of detection, application, and budgetary needs. Particles in the light beam at the time of aProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. There is currently no charge for using this plate reader. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. These lab workhorses are used in drug discovery. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Sale price $18,499. Molecular Devices microplate readers. Reader Documentation Version No. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. We would like to show you a description here but the site won’t allow us. Most papers they cultured bacteria to OD600>0. Shop all used microplate readers and parts from a variety of manufacturers. SpectraMax i3x Multi-Mode Microplate Re. the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to. Optical absorbance was measured at 734 nm on a SpectraMax M5. SpectraMax Injector Cartridge with SmartInject™ Technology. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Fluorescence Microplate Reader Comparison Chart. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. • Tel. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The plate is set up for reading and images are acquired according to specified parameters. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices. 00. The chamber. Select the Driver tab. Molecular Devices SpectraMax Plus 384. It also integrates with the. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. View the Molecular Devices website. ". The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. 6: bioNova científica, s. 7. * Nunc. SpectraTest ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orThe Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Multimode Reader Comparison Chart. Our absorbance plate readers feature our PathCheck Sensor technology and. 2. 04 to 3. The M5e Reader is easily automated. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. Wide range of applications. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Copia stocks a full line of used microplate readers. No accessories are needed and SoftMax® Pro Software. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. ** Please reference item #181108-12454-08 when. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. 72 MB. $6,649. 010 OD 384-Well Plate,. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. com 10. 383 inches whereas the maximum allowed is 5. 2. If no sound is heard during initialization, contact technical support. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 FlexStation® 3 Multi-Mode Microplate Reader. 12. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 3) At the bottom of the strip, click on "Show. The Infinite and Spark multimode microplate readers are modular and upgradeable. Unplug the power cable from the back of the instrument. 7°C (gradient heating design). Step 4. The performance of these two modes is not comparable to dedicated luminescence or timeresolved fluorescence instruments, or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Thank you!. We empower scientists to advance discovery Automated, end-to-end solutions span research disciplines to help scientists discover, scale, and industrialize biology. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 1. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 VAL014, right table) assays on the SpectraMax M5, M5e, or FlexStation 3 reader are shown below. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. SpectraMax® M5 Multi-Mode Microplate Re. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Description 1. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 1. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9660-665 Eskdale Road. Cells in each image are identified by SoftMax Pro Software and cell-by-cellit is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. TR-FRET assay. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. An integration time of 1 second was used for each reader. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. Restart the system. For example, microplates containing different samp les can be read using the same orThe Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). edu, 814-863-3503. Spectramax M5. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. With optimized reagents, option to operate the. 6 for further experiment, so I'm confused which i would choose for OD value determination. SpectraMax M5 reader SpectraMax M5e. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. There is currently no charge for using this plate reader. 1321N1, a human astrocytoma cell line, expresses. REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Third this recommendation. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. spr SpectraMax Plus 384. Spectramax Softmax Pro 6. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. book Page 1 Wednesday, July 7, 2010 2:09 PMThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 87. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. 2. It was then read from the top and bottom on the SpectraMax iD3 reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. 6 explains the lower values on the LmaxII384 plate reader. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Supersedes 1. Here the delta mP was 336, and Z’ factor was 0. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Choose from a three- (M3), four- (M4), orProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Recertification is recommended every two years. . Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Type Clear: Add to Cart. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. Molecular Devices. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. plate type, read area and number of images per well, plus specify positive/negative wells. Microplate Readers. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Also refer to this Multimode Reader Comparison Chart. Fluorescence intensity was measured using a. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. FlexStation II 384. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. Compatible Fluorescence Polarization Plate Readers. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax ® i3x. 2. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. SpectraMax Spectrofluorometers. Supersedes 1. Plate Reader with 5 modes of detection, UV-Vis (Abs), Fluorescence (FI) , Lumi, TRF, F Polarization, with current validations, fully inspected. Your all-access pass to your next breakthrough. The BD plate dimensions are 5. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). IMAP assays are based on binding of. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. UV/Vis Photometric Performance: Wavelength Range. To update the driver. Reader Documentation Version No. SpectraTest ABS2 Absorbance Validation Package performance tests. Wokingham, Berkshire RG41 5TS United Kingdom. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. *This plate definition in SoftMax Pro Software is the same for black and white plates. I accidentally left a plate in my lab's M5 SpectraMax plate reader overnight. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 314 low lamp level warning. Catalog Number: M5. 2018. The dedicated luminescence PMT has extremely low background counts and superior performance in the blue wavelengths. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The assay window spanned four orders of magnitude. Figure 10: The Plate section Template Editor, showing the series set up. Wait 20 seconds. Abs). Temperature Regulation. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. Select Start>Control panel>Device Manager. ZERO BIAS - scores, article reviews, protocol conditions and morePage 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Optics. 1. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. With optimized reagents, option to operate the readers. Dynamic Range. New Life Scientific backs all orders with a 90-day warranty. 4. This reader's dual monochromators for. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. flexibility of monochromator-based systems. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. View online or download Molecular devices SpectraMax M5 User Manual. FlexStation II. This. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The chamber. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. HTRF is a. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. SpectraMax M5 microplate reader. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Multimode Readers Comparison Chart. Uncontrolled Copy for Training and Reference Purposes Only . Your applications, your modes, your choice. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. For more information, please visit us at Plate-Based Assays. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. Reader Documentation Version No. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceMolecular Devices | Life Science Applications | Bioanalytical. NIST-traceable solution for validating the SpectraMax 340PC384 system. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package.